Miscellaneous Molecular Biology Reagents
Invitrogen™ Guanidine Thiocyanate
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents.
Invitrogen™ UltraPure™ Glycerol
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.
Thermo Scientific™ Aminoallyl-UTP Solution (50 mM)
Thermo Scientific Aminoallyl-UTP (5-[3-aminoallyl]-2'-uridine-5'-triphosphate) is supplied as 50 mM aqueous solution titrated to pH 7.0 with NaOH.
Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)
In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing.
Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Invitrogen™ MagMAX™ Wash Solution 2 Concentrate
Invitrogen MagMAX Wash Solution 2 Concentrate is a component of all the MagMAX Isolation Kits that may be purchased separately.
Polysorbate 20
CAS: 9005-64-5 Molecular Formula: C26H50O10 Synonym: Polyoxyethylene(20)sorbitan monolaurate
Thermo Scientific™ Formamide
Prepare hybridization cocktails and various denaturing solutions used with nucleic acids using this highly pure formulation of formamide denaturant.
Promega Herring Sperm DNA
Produces fragments with a size range of 80% between 100-3,000 base pairs; free of DNase or RNase activity.
Thermo Scientific™ EDTA
Chelate alkaline earth metals. Thermo Scientific™ Pierce EDTA is highly purified for use in a variety of laboratory methods.
Poloxamer 188
Molecular Formula: C5H10O2 Synonym: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
Thermo Scientific Chemicals Dodecyl sulfate, sodium salt, 99%, for biochemistry
CAS: 151-21-3 Molecular Formula: C12H25NaO4S,C12H25NaO4S
Thermo Scientific™ MassRuler DNA Loading Dye (6X)
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X MassRuler DNA Loading Dye.
Thermo Scientific™ Tris
Perform a variety of protein and molecular biology methods. Thermo Scientific™ Pierce™ Tris is a standard Tris buffering reagent available in a 1kg pack.
MilliporeSigma™ Chemicon™ Dextran Sulfate 50% Solution
Used as additive in probe hybridization solutions.