Miscellaneous Molecular Biology Reagents
Invitrogen™ ATP Solution (10 mM)
This Invitrogen ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript™ Kits and is supplied in one tube containing 100 μL.
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Thermo Scientific™ Co-factors
Thermo Scientific enzymes for in vitro transcription are designed for efficient and reliable RNA synthesis. These enzymes cover the entire synthesis workflow from template preparation to post-transcriptional modifications, ensuring optimal performance and high yields of synthesized RNA.
Thermo Scientific™ X-Gal
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
Invitrogen™ UltraPure™ Cesium Chloride, Optical Grade
UltraPure™ Cesium Chloride, Optical Grade is specifically prepared for use in density gradients.
Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution
UltraPure Guanidine Isothiocyanate Solution consists of 4M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA.
Invitrogen™ UltraPure™ Formamide
UltraPure Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization.
Invitrogen™ PureLink™ RNase A (20 mg/mL)
PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.
Thermo Scientific™ DNA Loading Dye & SDS Solution (6X)
Perform agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins with this DNA loading dye containing SDS.
Thermo Scientific™ Agarose II (Low Melt)
Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.
Thermo Scientific™ Tris
Perform a variety of protein and molecular biology methods. Thermo Scientific™ Pierce™ Tris is a standard Tris buffering reagent available in a 1kg pack.
Invitrogen™ X-Gal
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.